International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (443) Last page : (448)
Article doi: http://dx.doi.org/10.18782/2320-7051.2552

Phylogenetic Analysis of the Bacteria Associated with the Fresh Water Micro-Alga Chlorella sp.

R. C. Patil* and B. L. Jadhav
Department of Microbiology, Bhavan’s College, Andheri (W), Mumbai- 400058
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai- 400098
*Corresponding Author E-mail: rcpatil68@gmail.com
Received: 5.02.2017  |  Revised: 16.02.2017   |  Accepted: 18.02.2017  

 ABSTRACT

The importance of the microbial association with fresh water algae is well known and reported by several researchers. However, majority of these studies focus on the interaction between the algae and the microbes and there are very few reports on the phylogenetic studies on these associated microbes. Therefore, in the present investigation, molecular phylogeny of the three different bacterial species associated with the Chlorella sp. was determined by amplifying and sequencing the genomic 16S rRNA region. The DNA sequences were analyzed using online BLASTn tool. The BLAST results were used to find out evolutionary relationship between these bacteria. The phylogenetic tree for these bacteria was constructed by using MEGA 5 software.

Key words: Polymerase chain reaction, 16S rRNA, Phylogenetic tree, Evolutionary relationship

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Patil, R.C. and Jadhav, B.L., Phylogenetic Analysis of the Bacteria Associated with the Fresh Water Micro-Alga Chlorella sp., Int. J. Pure App. Biosci.5(3): 443-448 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2552