International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (225) Last page : (230)
Article doi: http://dx.doi.org/10.18782/2320-7051.2737
Comprehensive Sequence Analysis of TLR Genes in Vechur Cattle
R. Lakshmi1*and K. K. Jayavardhanan2
1Research Fellow, Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai – 600 007
2Professor and Head, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Thrissur, Kerala – 680 651
*Corresponding Author E-mail: lakshmivetbio@gmail.com
Received: 22.03.2017 | Revised: 5.04.2017 | Accepted: 6.04.2017
ABSTRACT
Vechur cow, an indigenous cattle variety of Kerala, is well adapted to the hot, humid tropical climate and high disease resistance. Breed characterization is a primary step in designing appropriate management and conservation programs of indigenous cattle. This research was carried out to characterize the Toll-like receptors (TLRs) of indigenous Vechur cattle breed. DNA was extracted from blood samples and total RNA from milk somatic cells was isolated using TRI reagent from Vechur cattle. Primers used to amplify the promoter region and mRNA of TLR2, TLR4 and TLR9 genes in Vechur cattle were designed from Bos taurus sequences. The amplified promoter and mRNA product were sequenced commercially by primer walking technique. The promoter region of TLR2 of Vechur cattle with the Bos taurus sequence showed 98 per cent similarity whereas TLR4 and TLR9 revealed 99 per cent similarity. TLR2 and TLR9 revealed variations for three sequence motifs. All three TLR mRNA sequences showed 99 per cent homology with Bos taurus sequence and exposed variations for 17 nucleotide in TLR2, 7 nucleotide in TLR4 and 5 nucleotide in TLR9 mRNA. The ectodomain of Vechur cattle displayed 10 LRRs for TLR2, 13 LRRs for TLR4 and 18 LRRs for TLR9. The primary structure of protein showed highest per cent of leucine amino acid for all three TLRs and alpha helix is the prominent secondary structure seen in all TLRs followed by beta turn and random coil. The tertiary structure was dominated by LRRs to form a solenoid protein domain. These data provide the most comprehensive view of TLRs in the Vechur cattle, and provide new opportunities to better understand the regulation of TLR genes in Vechur cattle.
Key words: Vechur, immune, TLRs, Promoters and mRNA
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Lakshmi, R. and Jayavardhanan, K.K., Comprehensive Sequence Analysis of TLR Genes in Vechur Cattle, Int. J. Pure App. Biosci.5(3): 225-230 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2737
