International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (989) Last page : (995)
Article doi: http://dx.doi.org/10.18782/2320-7051.2914
Genetic Analysis of Cyprinid Species from Kashmir Valley Based on Random Amplified Polymorphic DNA
Asifa Wali1*, Syed Mudasir Ahmad2, Imran Ganai3 and Masood-ul Hassan Balkhi1
1Faculty of Fisheries Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Rangil, Ganderbal Srinagar -19006, India
2Division of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama. Sher-e- Kashmir University of Agricultural Sciences and Technology, Kashmir, Shuhama Srinagar -19006, India
3Division of Animal nutrition, Faculty of Veterinary Sciences and Animal Husbandry, R.S.Pura.
Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, India
*Corresponding Author E-mail: asifawali@gmail.com
Received: 26.04.2017 | Revised: 8.05.2017 | Accepted: 10.05.2017
ABSTRACT
The random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 3 populations of cyprinid species Cyprinus carpio var. communis, Cyprinus carpio var. specularis and Carassius carassius collected from different locations of Kashmir, India. Twenty five samples for each of the three species collected from different locations were used for this study. Of the 12 random primers used 8 generated the polymorphism and showed 82.23% polymorphism. The study showed RAPD marker efficiently helped in inter- and intra- species variation of the three species. The unweighted pair group method with averages (UPGMA) was used to construct dendrogram for RAPD which resulted in two clusters, one containing C. C. Communis and C. C. specularis and the other included the C. carassius species. The Phylogenetic analysis demonstrated that scale carp (Cyprinus carpio communis) is closest to mirror carp (Cyprinus carpio specularis) than from crucian carp (Crassius carassius).
Keywords: Cyprinid, Genetic variation, RAPD, Polymorphism.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Wali, A., Ahmad, S.M., Ganai, I. and Balkhi, M.H., Genetic Analysis of Cyprinid Species from Kashmir Valley Based on Random Amplified Polymorphic DNA, Int. J. Pure App. Biosci.5(3): 989-995 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2914
