International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (899) Last page : (904)
Article doi: http://dx.doi.org/10.18782/2320-7051.4030

Optimization of Trehalase in Aspergillus niger

Deepti Chrungu*, Sukesh Chander Sharma and Akhtar Mahmood
Department of Biochemistry, Panjab University, Sector-25, Chandigarh, India -160014, India
*Corresponding Author E-mail: deeptichrungubch@gmail.com
Received: 12.06.2017  |  Revised: 23.06.2017   |  Accepted: 24.06.2017  

 ABSTRACT

Aspergillus niger produces enzyme trehalase, which is responsible for conversion of trehalose to glucose. Conidia from 15-day old culture were used for optimizing the trehalase activity using different cellulosic media and different carbohydrates; further its distribution was studied. Enzyme activity was assayed by estimating glucose released using 3,5-Dinitrosalicylic acid. Highest trehalase activity was reported in presence of oatmeal as compared to corn and walnut sources. Further, highest trehalase activity was present as extracellular protein. High levels of enzyme activity were obtained in presence of 1 % starch. There was nearly 80% increase in specific activity of the enzyme in presence of starch. Specific activity of trehalase enzyme in presence of starch was 46.62 ± 1.39 µmol/min/mg.

Key words: Aspergillus niger, Conidia, Optimization, Trehalase.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Chrungu, D., Sharma, S,C. and Mahmood, A., Optimization of Trehalase in Aspergillus niger, Int. J. Pure App. Biosci.5(3): 899-904 (2017). doi: http://dx.doi.org/10.18782/2320-7051.4030