International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (740) Last page : (748)
Article doi: http://dx.doi.org/10.18782/2320-7051.2558
Evaluation of Germplasm and Biocontrol Agents against Bacterial Canker (Clavibacter michiganensis Subsp. Michiganensis) of Tomato in Himachal Pradesh
Gurvinder Singh1*, Narender K. Bharat1, Ramesh K. Bhardwaj2 and Shiv Pratap Singh2
1Department of Plant Pathology, 2 Department of Vegetable Science, Dr. Y.S. Parmar University of Horticulture & Forestry, Nauni, Solan (HP)-173 230, India
*Corresponding Author E-mail: gurvinders50@yahoo.com
Received: 10.02.2017 | Revised: 21.02.2017 | Accepted: 22.02.2017
ABSTRACT
Bacterial canker (Clavibacter michiganensis subsp. michiganensis) of tomato (Solanum lycopersicum) is a newly emerging bacterial disease in mid hills of Himachal Pradesh, India. The disease is causing huge losses to the tomato production in this region. Under the present investigations, the bacterial canker pathogen was isolated in pure culture. For inculcation of bacteria in young tomato plants for pathogenicity testing and germplasm screening, methods like syringe inoculation of leaves and branches, leaf clipping and stem inoculation with toothpick were adjudged effective. Out of 18 tomato lines/ cultivars tested against the pathogen, none was found resistant to the disease after artificial inoculation in pot culture. However, seven lines viz., EC – 521086, EC – 521074, EC – 251649, EC – 25265, EC – 521054, EC – 35322 and EC – 2791 exhibited moderately susceptible reaction and rest of the lines/ cultivars were either susceptible or highly susceptible. Among five bacterial biocontrol agents tested against the bacterial pathogen in vitro, Pseudomonas flourescens strain PfS-1 was found effective in inhibiting the growth of the pathogen. This biocontrol agent when inoculated through seed, improved germination and delayed the expression of disease symptoms in pot culture.
Key words: Bacterial canker of tomato, disease management, biological control, germplasm screening
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Singh, G., Bharat, N.K., Bhardwaj, R.K. and Singh, S.P., Evaluation of Germplasm and Biocontrol Agents against Bacterial Canker (Clavibacter michiganensis Subsp. Michiganensis) of Tomato in Himachal Pradesh, Int. J. Pure App. Biosci.5(3): 740-748 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2558
