International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (774) Last page : (778)
Article doi: http://dx.doi.org/10.18782/2320-7051.2697
A Study on Plasmid Borne Antibiotic Resistance Patterns in Escherichia coli
Niyaz A Dar1, Shahid A Padder2*, Zahoor A Bhat3 and Abdul Q Ganai3
1Division of Plant Biotechnology, 2Division of Plant Pathology, 3Division of Soil Science SKAUST-K Shalimar Srinagar Kashmir (J&K) 190025
*Corresponding Author E-mail: aafan.tavkeer@gmail.com
Received: 17.03.2017 | Revised: 28.03.2017 | Accepted: 30.03.2017
ABSTRACT
The objective of this study was to determine if E.coli plasmid borne genes for antibiotic resistance. This study was carried out at SKAUST-K for five different strains of E.coli : E1, E2, E3, E4 and E5. For monitoring trends in antibiotic resistance prevalence in E.coli, isolates from five healthy persons were analyzed in laboratory using Luria Broth agar plates containing respectively azithromycin, cefadroxil, cefixime, norfloxacin & amoxycllin at breakpoint concentrations with DH5α as host for plasmid transformation. The mean age of the volunteers was 30 years. Cefixime resistance was higher in the range 75-80% however maximum zone of inhibition was recorded against azithromycin. Of the entire isolates maximum zone was observed in E1 E.coli isolate. All five isolate samples of E.coli shows inducible mode of detoxification mechanism for antibiotics. Using growth inhibitory studies and also plasmids obtained from sample strains resolved at same position on agarose gel determining that they were of similar size. In the population studies, antibiotic resistance in E.coli is emerging for norfloxain & azithromycin but is higher for the primitive drugs like cefixime, cefadroxil & amoxycllin.
Key words: Antibiotic resistance, emerging resistance, azithromycin, Norfloxacin DH5α.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Dar, N.A., Padder, S.A., Bhat, Z.A. and Ganai, A.Q., A Study on Plasmid Borne Antibiotic Resistance Patterns in Escherichia coli, Int. J. Pure App. Biosci.5(2): 774-778 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2697
