International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (541) Last page : (548)
Article doi: http://dx.doi.org/10.18782/2320-7051.2721
Identification and Molecular Diversity Analysis of Bacillus subtilis from Soils of Western Ghats of Karnataka using 16S rRNA Bacterial Universal Primers
Bhimanagoud Kumbar1*, Riaz Mahmood1 and Nagesha Somakalapalli Narasimhappa2
1Department of P G studies and Research in Biotechnology, Jnanasahyadri, Kuvempu University, Shankaraghatta, Shimoga – 577451 Karnataka, India
2Department of Biotechnology, College of Agriculture, Karekere, Hassan – 573225 Karnataka, India
*Corresponding Author E-mail: kiranmk72@gmail.com
Received: 19.03.2017 | Revised: 2.04.2017 | Accepted: 5.04.2017
ABSTRACT
Bacillus subtilis (B. Subtilis) is a model organism and to study the chromosomal replication and cell differentiation in bacteria.B.subtilis is the gram positive bacterium and the strains of this bacterium were isolated from soils of Western Ghats of Karnataka and characterized by molecular and morphological methods as a result 13 new B.subtilis strains were identified with reference strain MTCC-2422 (Microbial Type Culture Collection and Gene Bank). The morphology study was carried out and isolated strains were further characterized by 16S rRNA bacterial Universal primers by PCR.Sequence analyses was carried out using NCBI BLASTn tool, sequences were submitted to the NCBI.The sequencing results were showed the variations in the nucleotide sequences. GC content of all the strains between 52 to 55.5% it shows that they are closely correlated to each other. In the phylogenetic tree two major groups were seen KU936344 and KU936333 formed one group and rest of the accessions formed another group which indicates that there is great degree of diversity. The reference strain MTCC-2422 is formed a group with KU936341. Isolates from Western Ghats of Karnataka are high degree of sequence similarity with different NCBI accession numbers. Present findings tell that the genetic diversity among newly isolated strains are closely related to each other.
Key words: Bacillus subtilis, 16S rRNA, Western Ghats of Karnataka, Molecular diversity, phylogenetic tree, NCBI accession numbers.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kumbar, B., Mahmood, R. and Narasimhappa, N.S., Identification and Molecular Diversity Analysis of Bacillus subtilis from Soils of Western Ghats of Karnataka using 16S rRNA Bacterial Universal Primers, Int. J. Pure App. Biosci.5(2): 541-548 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2721
