International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (118) Last page : (128)
Article doi: http://dx.doi.org/10.18782/2320-7051.2612
Evaluation of the Anthelmintic Activity (in- vitro) of Neem Leaf Extract-Mediated Silver Nanoparticles against Haemonchus contortus
Avinash. B1*, Supraja. N2, Charitha. V.G1, Adeppa. J1 and Prasad. T.N.V.K.V2
1Department of Veterinary Parasitology, NTR CVSc, Gannavaram, Sri Venkateswara Veterinary University, Tirupati-517502, A.P. India
2Acharya N. G. Ranga Agricultural University, Regional Agricultural Research Station, Tirupati-A.P. India
*Corresponding Author E-mail: avinashbodaballa@gmail.com
Received: 18.02.2017 | Revised: 1.03.2017 | Accepted: 5.03.2017
ABSTRACT
In vitro anthelmintic activity of aqueous Azadirachta indica (neem) leaf extract mediated silver nanoparticles (AgNPs) against Haemonchus contortus was assessed. The prepared AgNPs were characterized using the techniques such as, ultraviolet–visible spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Dynamic light scattering (DLS) and TEM (transmission electron microscopy). UV–VIS spectrum of the aqueous medium containing silver nanoparticles showed absorption peak at 410 nm. Fourier transform infra-red spectroscopic analysis revealed that primary amine groups, alcohols and phenols in combination with the proteins present in the leaf extract are responsible for the reduction and stabilization of the AgNPs. XRD micrograph indicated the face centered cubic structure of the formed AgNPs. The morphological studies including size indicating that the prepared AgNPs were relatively spherical in shape with an average size of 25 nm. The hydrodynamic diameter (99.5 nm) and zeta potential (-172.4 mV) were measured using the dynamic light scattering technique.
In-vitro anthelmintic tests like adult motility test (AMT) and egg hatch assay (EHA) were performed at varying Seven concentrations of AgNPs and aqueous extract were used for EHA and AMT. Crude aqueous-nem leaf extract @ 6.0, 3.0, 1.5, 0.75, 0.375, 0.1875 and 0.09375 mg/mL, AgNPs (0.17, 0.085, 0.0425, 0.02125, 0.01062, 0.0053 and 0.00265mg/ mL .The highest concentration induced 100% egg hatch inhibition. For EHA the IC50and IC 90 values were 0.005mg/ml and 0.066 mg/ml for AgNPs. IC50 and IC90 values for EHA were 0.665 mg/ml and 3.569 mg/ml for neemextract. IC50 and IC90 values 1.065 and 2.382 mg/ml for albendazole.
For AMT also seven different concentrations were tested and Piperazine adepate (12 mg/ml) was used as positive referral and PBS as negative control. At higher dose, worms were completely paralyzed within one minute of exposure to AgNPs and completely paralyzed within 5 min for neem extract. As the concentration of the compounds decreased the degree of immobilization got delayed. The total time taken for mortality of worms with reference drug piperazineadepate (12mg/ml) was 6 min. The overall findings of the present study show that the AgNPs have potent ovicidal and wormicidal activity.
Key words: Silver nanoparticles, Azadirachta indica, Haemonchus contortus, Adult motility test, Egg hatch assay
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Avinash, B., Supraja, N., Charitha, V.G., Adeppa, J. and Prasad, T.N.V.K.V., Evaluation of the Anthelmintic Activity (in- vitro) of Neem Leaf Extract-Mediated Silver Nanoparticles against Haemonchus contortus, Int. J. Pure App. Biosci.5(2): 118-128 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2612
