International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (107) Last page : (117)
Article doi: http://dx.doi.org/10.18782/2320-7051.2539
Green Synthesis and Characterization of Silver Nanoparticles from Momordica charantia Fruit Extract: Study of Antimicrobial Activities
N. Supraja1, B. Avinash2 and T. N.V.K.V Prasad1*
1Acharya N. G. Ranga Agricultural University, Regional Agricultural Research Station, Tirupati-A.P. India
2College of Veterinary Science Tirupati, Sri Venkateswara Veterinary University, Tirupati
*Corresponding Author E-mail: tnvkvprasad@gmail.com
Received: 2.02.2017 | Revised: 15.02.2017 | Accepted: 16.02.2017
ABSTRACT
The use of environmentally benign materials such as plant extract for the synthesis of silver nanoparticles (AgNPs) offers numerous benefits of eco-friendliness and compatibility for pharmaceutical and other biomedical applications. In this study, we have used Momordica charantia fruit extract as a reducing agent for the synthesis of AgNPs. Characterization of the AgNPs was done by UV-Visible Spectroscopy, Scanning Electron Microscopy (HR-SEM), X-ray diffraction (XRD), Dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). An intense surface plasmon resonance band at 450nm in the UV–visible spectrum clearly revealed the formation of AgNPs. SAED and XRD patterns confirmed the presence of highly crystalline and face-centered cubic structure AgNPs and they were generally found to be spherical in shape with variable size ranging from 10 to 50nm, as evident by SEM. Phenolic compounds present in Momordica charantia fruit extract were mainly responsible for the reduction and the stabilization of AgNPs. The biosynthesized AgNPs exhibited a strong antimicrobial activity against gram positive and negative bacteria, Fungi.
Key words: Green Synthesis, Silver Nanoparticles, Momordica Charantia Fruit Extract, Antimicrobial Activity.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Supraja, N., Avinash, B. and Prasad, T. N.V.K.V., Green Synthesis and Characterization of Silver Nanoparticles from Momordica charantia Fruit Extract: Study of Antimicrobial Activities, Int. J. Pure App. Biosci.5(1): 107-117 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2539
