International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (787) Last page : (793)
Article doi: http://dx.doi.org/10.18782/2320-7051.2656
Assessment of Genetic Diversity among the Accessions of Jeera (Nigella Sativa L.), from Afghanistan and India using Molecular Traits
Sayed Esmail Emran1*, Rashad Ahmad Sherzad2, Sayed Ali Yaqoobi3, Sayed Ali Askar Musavi4
1Department of Plantation, Spices, Medicinal, and Aromatic Crops, College of Horticulture, UHS Campus, Bengaluru, Karnataka, India
2Department of postharvest technology, College of Horticulture, UHS Campus, Bengaluru, Karnataka, India
3Department of Horticulture, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran
4Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
*Corresponding Author E-mail: emran50000@gmail.com
Received: 6.03.2017 | Revised: 18.03.2017 | Accepted: 19.03.2017
ABSTRACT
Estimation of genetic diversity is essential for breeding programs and for the conservation of genetic resources. Assessment of the genetic diversity in black cumin is therefore, of crucial importance for its genetic improvement. Molecular techniques have also had critical roles in studies of phylogeny and species evolution, and have been applied to increase our understanding of the distribution and extent of genetic variation within and between species. RAPD primers were used for molecular diversity analysis of 12 accessions representing diverse morphological clusters. The number of bands produced by each primer ranged from 1 to 11. The highest number of loci was amplified in I.15 and BH.2 primers and the lowest was in I.18, the gene diversity observed ranged from 0.00 in primer I.14 to 0.42 in primer I.1 with a mean gene diversity of 0.27, the Polymorphic information contents (PIC) of the 11 RAPD primers that were used varied between 0.00 and 0.35. Average PIC was 0.22. The genotypes from Afghanistan and India had similar similarity index values within each group (or country) than between them suggesting that short geographical isolation has low influence on the genetic diversity of the genotypes.
Key words: Nigella sativa, Afghanistan, India, RAPD, Diversity, PIC
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Emran, S.E., Sherzad, R.A., Yaqoobi, S.A. and Musavi, S.A.A., Assessment of Genetic Diversity among the Accessions of Jeera(Nigella Sativa L.), from Afghanistan and India using Molecular Traits, Int. J. Pure App. Biosci.5(2): 787-793 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2656
