International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (744) Last page : (752)
Article doi: http://dx.doi.org/10.18782/2320-7051.2587
Screening of Efficient Ethanol Tolerant Yeast Strain for Production of Ethanol
Ningaraj Dalawai1, Krupa, K. N.1, Siddhesh Nadkarni2, Subhash Bharani1* and Harinikumar, K. M.1
1Department of Plant Biotechnology, UAS, GKVK, Bengaluru
2Department of Plant Breeding and Genetics, College of Agriculture, Vellayani Trivandrum
*Corresponding Author E-mail: subash4586@gmail.com
Received: 13.02.2017 | Revised: 25.02.2017 | Accepted: 26.02.2017
ABSTRACT
An efficient method for conversion of biomass into fuel is by ethanol production because ethanol is an economically as well as environmentally friendly fuel. Ethanol has the advantages of being renewable, cleaner burning and produces no greenhouse gases. Hence Ethanol tolerance investigation was carried out with yeast strains by isolating from their natural habitats and screened them for ethanol tolerance and ethanol production. Out of 40 microbial culture 10 were identified as Saccharomyces strains based on colony type and budding characters. Saccharomyces species were screened for their ability to tolerate different ethanol concentrations from 0-24 %. Growth in different ethanol concentrations varied from one strain to another. Some Yeast strains showed tolerance level from 7-15 %. Even though some strains had tolerance at 15 to 16 % but the growth was less. Yeast from Sugarcane juice and grapes showed highest tolerance, whereas from pineapple least tolerance was observed among 10 isolates. Ten Saccharomyces strains were subjected to SSR and ADH specific primer analysis using eight primers. Cluster diagram was divided into 3 major clusters and 4 sub clusters, two major cluster showing 25 % dissimilarity. There was correlation between ethanol tolerance and genetic relatedness shown by SSR and ADH specific primer analysis.
Key words: Bioethanol, S. cerevisiae, Bioenergy, Sustainable, Renewable, Biomass
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Dalawai, N., Krupa, K. N., Nadkarni, S., Bharani, S. and Harinikumar, K. M., Screening of Efficient Ethanol Tolerant Yeast Strain for Production of Ethanol, Int. J. Pure App. Biosci.5(1): 744-752 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2587
