International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (674) Last page : (678)
Article doi: http://dx.doi.org/10.18782/2320-7051.2459
Random Amplified Polymorphic DNA (RAPD) Analysis of Released Varieties and Hybrids of Pigeonpea [Cajanus cajan (L.) Millspaugh] of Gujarat
Poonam Thakar1*, R.M. Chauhan2, and Prashant Joshi3
1,2Department of Agricultural Biotechnology, C. P. College of Agriculture,
3Department of Food Science and Nutrition,
S.D. Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India
*Corresponding Author E-mail: thakar.poonam08@gmail.com
Received: 14.01.2017 | Revised: 25.01.2017 | Accepted: 26.01.2017
ABSTRACT
For diversity analysis of pigeonpea molecular marker used to study 11 diverse genotypes of pigeonpea using 12 each of RAPD primers used to determine varietal/hybrid specific informative molecular markers for precise identification of varieties and hybrid of pigeonpea. DNA was extracted as per CTAB method with minor modification and quality as analysed on nanodrop, the absorbance ratio of DNA at A260/A280 and finally with electrophoresis. Comparatively, RAPD primers generated 97 amplicons with an average 7.46 amplicons per RAPD primers. RAPD primers classified the eleven genotypes of pigeonpea into two different clusters with lot of variation in number and inclusion of genotypes in sub groups. These techniques indicated genotype GT -100 to be distantly apart from genotype GT 288 A, yet comparing other set of genotypes, GT-101 was evinced closer to GT-102 in RAPD analysis.
Key words: RAPD, DNA, Pigeonpea, Hybrids
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Thakar, P., Chauhan, R.M., and Joshi P., Random Amplified Polymorphic DNA (RAPD) Analysis of Released Varieties and Hybrids of Pigeonpea [Cajanus cajan(L.) Millspaugh]of Gujarat, Int. J. Pure App. Biosci.5(1): 674-678 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2459
