International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (667) Last page : (673)
Article doi: http://dx.doi.org/10.18782/2320-7051.2611

Isolation of Crude Proteases from Freshwater Fishes Catla catla and Labeo rohita: Optimizing the Hydrolysis Conditions of Crude Proteases

Pavan Kumar D.*, K. Elavarasan and B. A. Shamasundar
Department of Fish Processing Technology, Karnataka Veterinary, Animal And Fisheries Sciences University, Bidar, College of Fisheries, Mangalore 575002, India
*Corresponding Author E-mail: pawankumardhara@gmail.com
Received: 18.02.2017  |  Revised: 26.02.2017   |  Accepted: 27.02.2017  

 ABSTRACT

Proteases from gastrointestinal (GI) tract of fresh water fish Catla catla and Labeo rohita have been studied. This study is prelude to use proteases form GI tract of fresh water fish for the production of gelatin protein hydrolysates. The optimum conditions for the crude proteases obtained from GI tract have been standardized. The optimum temperature for the activity of enzymes from two species of fish found to be 55 oC and hydrolysis time of 15 min. The pH optima for the proteases from catla were found to be 10.5 and that from rohu was 8.5 and can be considered as alkaline proteases. The substrate used to assay the proteolytic activity was casein. The enzymes from two species had number of subunits in molecular weight range of 6.5–97 kDa as revealed by electrophoretic mobility under reduced conditions.

Key words: Freshwater fish, digestive proteases, proteolytic activity, SDS-PAGE

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar P.D., Elavarasan, K. and Shamasundar, B.A., Isolation of Crude Proteases from Freshwater Fishes Catla catla and Labeo rohita: Optimizing the Hydrolysis Conditions of Crude Proteases, Int. J. Pure App. Biosci.5(1): 667-673 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2611