International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (254) Last page : (260)
Article doi: http://dx.doi.org/10.18782/2320-7051.2591
Validation of Molecular Markers Linked to Fusarium Wilt Resistance in Chickpea Genotypes
Maisuria, Hemanshukumar Jekishandas1, Patel, Rameshbhai Mohanlal2 and Suthar, Kirankumar Parshottambhai3*
1Department of Agricultural Biotechnology, Anand Agricultural University, Anand 388 110, Gujarat, India
2ASPEE SHAKILAM Agricultural Biotechnology Institute, Navsari Agricultural University, Surat 395 007, Gujarat, India
3Department of Plant Molecular Biology and Biotechnology, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari 396 450, Gujarat, India
*Corresponding Author E-mail: kpsbiotech@rediffmail.com
Received: 13.02.2017 | Revised: 24.02.2017 | Accepted: 25.02.2017
ABSTRACT
Chickpea (Cicer arietinum L.) is third most important legume crop called as poor men's meat due to good source of protein. However, the productivity of chickpea is limited due to fungal disease wilt, caused by Fusarium oxysporum f. sp. ciceris. The resistance breeding aimed to developed disease resistance variety and Marker Assisted Selection can gear up conventional breeding approaches. The present research work was appointed for validation of different markers viz., CS-27, UBC-170, CS-27A, OP-U17-1, UBC-825, TA-59, TA-96 and TR-19 previously reported to be linked to wilt disease either susceptibility or resistance in chickpea. Among susceptibility linked markers CS-27700 and UBC-8251200 validated for all susceptible genotypes, whereas CS-27A700 was not validated only in susceptible genotypes, GG-4 while OP-U17-11014 was not validated in GG-4. The resistance linked markers UBC-170500 was validated in all resistant genotypes. The markers TA-59258 and TA-96265 did not give specific amplicon in resistance genotypes GG-1, GG-2 and annigeri, GG-1, respectively while TR-19227 did not amplify specific product in resistance genotypes annigeri, GG-1 and GG-2. Out of eight markers studied, three markers CS-27700, UBC-8251200 and UBC-170500 were validated in fifteen diverse chickpea genotypes and found to be consistence for marker assisted characterization. These markers could be utilized in future for maker assisted breeding for wilt resistance in chickpea.
Key words: Cicer arietinum L., Fusarium wilt, Molecular markers, Resistance, Susceptibility.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Maisuria, H.J., Patel, R.M. and Suthar, K.P., Validation of Molecular Markers Linked to Fusarium Wilt Resistance in Chickpea Genotypes , Int. J. Pure App. Biosci.5(1): 254-260 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2591
