International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (99) Last page : (103)
Article doi: http://dx.doi.org/10.18782/2320-7051.2453
Expression of TLR4 Gene in Spontaneous Bovine Sub-Clinical and Clinical Mastitis Caused by Escherichia coli
R. Lakshmi1* and K.K. Jayavardhanan2
1Research Fellow, Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 007
2Professor and Head, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Thrissur, Kerala - 680651, India
*Corresponding Author E-mail: lakshmivetbio@gmail.com
Received: 13.01.2017 | Revised: 22.01.2017 | Accepted: 23.01.2017
ABSTRACT
Escherichia coli infection of the bovine mammary gland can result in a spectrum of sub-clinical and clinical infection to a severe systemic mastitis disease. E. coli appear to mostly circumvent the host immune response through the signalling of Toll-Like Receptors (TLRs). Understanding the expression of TLR genes in bovine is particularly important to develop strategies for the prevention of mastitis. This study characterized the expression of an important TLR4 gene in spontaneous bovine sub-clinical and clinical mastitis caused by E. coli. The mRNA expression of TLR4 gene in sub-clinical mastitis was higher (6.55 fold) than clinical case (2.88) when compared to normal bovine case. Relative difference in the expression of TLR4 gene in sub-clinical and clinical mastitis was found to be significant (P ≤ 0.01). E. coli elicits a strong and earlier response in sub-clinical mastitis, mainly through TLR4, and hence the expression of TLR4 is relatively higher at this stage compared to clinical stage. During early stage of infection the expression of TLR4 gene was at high level, therefore most of the sub-clinical mastitis subsided by itself without precipitating into clinical mastitis. This study sheds light into the innate immune system represents the first line of defense in the host response to infection and is poised to immediately recognize and respond to the earliest stages of infection.
Key words: TLR4, expression, mastitis and E. coli
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Lakshmi, R. and Jayavardhanan, K.K., Expression of TLR4 Gene in Spontaneous Bovine Sub-Clinical and Clinical Mastitis Caused by Escherichia coli, Int. J. Pure App. Biosci.5(1): 99-103 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2453
