International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (844) Last page : (848)
Article doi: http://dx.doi.org/10.18782/2320-7051.2476
Detection of GFP Expression and Colonization of Wheat by two Endophytic Bacteria tagged with GFP Gene
Disheeta L. Akbari1* and L. F. Akbari2
1Department of Biotechnology and 2Department of Plant Pathology,
Junagadh Agricultural University, Junagadh-362001, Gujarat, India
*Corresponding Author E-mail: disheetaakbari@gmail.com
Received: 19.01.2017 | Revised: 30.01.2017 | Accepted: 1.02.2017
ABSTRACT
The two endophytic bacteria had 92% similarity with Pseudomonas synxantha strain NBRC103159 and 96% with Pseudomonas cedrina strain CFML96-198 were identified using 16S ribosomal DNA sequence and isolated from stems of Eleusine indica. Its endophytic colonization were investigated microscopically using green fluorescent protein introduced by vector pGLO containing ampicillin resistance gene and arabinose sugar. The strains were able to colonize wheat tissues and localized in the roots, stems and leaves.
Key words: Pseudomonas spp., pGLO vector, GFP, Fluorescent microscopy, Wheat
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Akbari, D.L. and Akbari, L.F., Detection of GFP Expression and Colonization of Wheat by two Endophytic Bacteria tagged with GFP Gene, Int. J. Pure App. Biosci.5(1): 844-848 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2476
