International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (777) Last page : (783)
Article doi: http://dx.doi.org/10.18782/2320-7051.2495
Estimation of Primary Metabolites and Phenols in the Seeds of Five Species of Calamus and their Role on Germination
Defedar Hanumanthaiah Tejavathi1*, Haranahalli Ramaiah Raveesha1, Rajashekaraiah Nijagunaiah1, Agrahara Chaluvegowda Lakshmana2, Ronur Venkateshalu Madhusudhan1 and Doddakythanahally Ramanatha Jayashree3
1Department of Botany, Bangalore University, Bangalore – 560 0056, India
2Principal Secretary (Retd.), Forest and Environment, Govt. of Karnataka, India
3Department of Biotechnology, M.S. Ramaiah College, Bangalore – 560 054, India
*Corresponding Author E-mail: tejavathi_hanu@yahoo.com
Received: 23.01.2017 | Revised: 5.02.2017 | Accepted: 8.02.2017
ABSTRACT
Background and Aim: The mass scale harvest of Calamus before flowering and fruiting resulted in the depletion of precious germplasms and shortage of required seed for further propagation. Seeds of Calamus species are recalcitrant and cannot withstand desiccation, a challenge for the storage. Correlation between biochemical traits and germination contribute to the better understanding of seed dormancy and to assess the germination capacity of the given taxon.
Materials and Methods: Carbohydrates, proteins, amino acids and phenol contents were estimated in the seeds of Calamus heugelianus, C. prasinus, C. nagabettai, C. thwaitesii and C. vattayila for the first time following standard biochemical methods.
Results: The contents have shown variations among the taxa studied. Carbohydrate content of 8.25 ± 0.04% is significantly high in the seeds of C. prasinus than other species studied. While higher content of proteins (8.92 ± 0.02%) and amino acids (9.36 ± 0.02%) were found in the seeds of C. heugelianus. The phenolics content was found to be more in the seeds of C. nagabettai than other taxa studied. The levels of all the studied bio constituents are minimum in C. vattayila. Effect of the storage macromolecules on germination behaviour of the seeds is discussed.
Key words: Calamus, Germination, Phenols, Primary Metabolites, Seeds.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Tejavathi, D.H., Raveesha, H.R., Nijagunaiah, R., Lakshmana, A.C., Madhusudhan, R.V. and Jayashree, D.R., Estimation of Primary Metabolites and Phenols in the Seeds of Five Species of Calamus and their Role on Germination, Int. J. Pure App. Biosci.5(1): 777-783 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2495
