International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (88) Last page : (93)
Article doi: http://dx.doi.org/10.18782/2320-7051.2424
Isolation, Identification and Characterization of Heavy Metal Resistant Bacteria from Industrial Affected Soil in Central India
Narendra Kumar Ahirwar1, Govind Gupta2, Ravindra Singh1, Vinod Singh2*
1Mahatma Gandhi Chitrakoot Gramodaya Vishwavidhyalaya, Chitrakoot, (M.P.), India
2Department of Microbiology, Barkatullah University, Bhopal, (M.P.), India
*Corresponding Author E-mail: vsingh3@rediffmail.com
Received: 20.12.2016 | Revised: 28.12.2016 | Accepted: 31.12.2016
Abstract
Microorganisms related technologies may provide an alternative or addition to conventional method of metal recovery or metal removal. The present study deals with isolation, identification and characterization of heavy metal resistant bacteria was isolated from industrial affected soils collected from different places at a depth of 0-15 centimeter, in and around Mandideep industrial areas, Raisen district, Central India. Initially, a total of 175 isolates were screened from industrial affected soil. The five isolates were selected based on high level of heavy metal resistances. On the basis of morphology, biochemical revealed that, the isolates were identified as Proteus vulgaris (MR1), Bacillus cereus (MR2), Bacillus decolorationis (MR3), Pseudomonas fluorescence (SS4) and Pseudomonas fluorescence (SS5). The soil isolates showed optimum growth at pH 7.0 and 30°C. The identified isolates were resistant to cadmium (Cd), nickel (Ni), lead (Pb), arsenic (As), and chromium (Cr). The minimal inhibitory concentration (MIC) of soil isolates against Cd, Cr, Ni, Pb and As was determined in solid media. The identified heavy metal resistant bacteria could be effective and useful for the bioremediation of heavy metal contaminated soil.
Key words:Contaminated Soil, Heavy metals, Soil Bacteria, and Bioremediation.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Ahirwar, N.K., Gupta, G., Singh, R. and Singh, V., Isolation, Identification and Characterization of Heavy Metal Resistant Bacteria from Industrial Affected Soil in Central India, Int. J. Pure App. Biosci.4(6): 88-93 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2424
