International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (59) Last page : (75)
Article doi: http://dx.doi.org/10.18782/2320-7051.2366
WSSV Transmission Studies on Polychaete Pereneris cultifera to Pacific White Shrimp SPF Litopenaeus vannamei in Captivity
R. Shalini, M.A. Badhul Haq*, A. Sajith Ahamed, P. Kumar, V. Sedhuraman and M. Nirosh Banu
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University,
Parangipettai - 608 502, Tamilnadu, India
*Corresponding Author E-mail: drhaqvirologyteam@gmail.com
Received: 2.09.2016 | Revised: 18.09.2016 | Accepted: 21.09.2016
Abstract
The preparation viral inoculums were used in this study was isolated from infected polychaete P. cultrifera from the experimental tank. Viral DNA was isolated from purified virions by treatment with proteinase K followed by phenol and chloroform extraction and dialysis against TE. The inoculum (viral DNA) was used to challenge WSSV-negative animals under experimental conditions. Triplicate tanks were maintained to each species shrimp with oral route and intramuscular injection inoculation. Tissues and hemolymph were collected from experimental shrimps for PCR analysis. Shrimps were sacrificed at 24h, 48h, 3, 6, 12, 18 and 25 days interval. PCR analysis showed the appearance of a prominent band of PCR amplified product of WSSV-DNA at 848 bp at 24 hr up to day 7 of post infection in the pleopods although these band 296 bp (200 copies) continued from day 8 to 12. On days 13 and 14 the product band observed as 650 bp (2000 copies) and an equal amount of product band of 910 bp (20,000) during the days 15 to 18. The same product band was ranged between 296 to 910 bp during 19 to 25 days in the L. vannamei shrimps which exposed to WSSV by oral route. No band was observed in the control group. Histological observation of low, moderate and severely WSSV infected L. vannamei shrimps were revealed degenerated cells characterized by basophilic intranuclear inclusions in the tissues of WSSV infected mid-gut gland, lymphoid organ, gill lamellae, gut epithelium tissue of L. vannamei on the 9th day of post-inoculum. The survival days of L. vannamei extends up to 23 days for oral rooted and 24 days for intramuscularly injected shrimps of which mortality rate on the concerning days was 93.30% and 90.0 % respectively.
Key words: PCR, Histopathology, WSSV, P. vannamei, P. cultrifera.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Shalini, R., Badhul Haq, M.A., Sajith Ahamed, A., Kumar, P., Sedhuraman, V. and Nirosh Banu, M., WSSV Transmission Studies on Polychaete Pereneris cultifera to Pacific White Shrimp SPF Litopenaeus vannamei in Captivity, Int. J. Pure App. Biosci.4(6): 59-75 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2366
