International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (159) Last page : (167)
Article doi: http://dx.doi.org/10.18782/2320-7051.2416

Micropropagation of Plumbago zeylanica L.: An Important Medicinal Plant of India

Rahul Vijay*, Jyoti Shukla and Rajesh Saxena
Madhya Pradesh Council of Science and Technology, Bhopal (M.P) India
*Corresponding Author E-mail: drrahulvijay001@gmail.com
Received: 1.12.2016  |  Revised: 14.12.2016   |  Accepted: 18.12.2016  

Abstract
Background and Aim: In-vitro effrct of various growth regulators on propagation of Plumbago Zeylanica L., a medicinally herbs of India was observed in this study. C. Paniculatus has been used in the various ancient traditional system.
Materials and Method: Healthy nodal segments of the herb was used as explants with basic MS medium for shoot initiation and multiplication containing various combinations of different growth regulators. MS full and 1/2MS were used for rooting of plantlets with 25 to 200 mg/l Activated Charcoal (AC).
Result: Maximum mean number of initiated plantlets 1.7 ± 0.48 with mean length 2.7 ± 0.82 were found in MS medium treated with lowest concentration of BA (0.44 µM), while maximum mean number of multiplied plantlets 19.4 ± 0.96 with mean length 6 ± 0.94 were found in MS medium treated with 4.44 µM. Maximum mean number of roods 12 ± 1.56 with Mean length 8.92 ± 0.75 was observed on 1/2MS medium with 125 mg/l activated charcoal. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.

Key words: Plumbago Zeylanica L., Micropropagation, Medicinal Plants, Plant Tissue Culture.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vijay, R., Shukla, J. and Saxena, R., Micropropagation of Plumbago zeylanica L.: An Important Medicinal Plant of India, Int. J. Pure App. Biosci.4(6): 159-167 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2416