International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (56) Last page : (63)
Article doi: http://dx.doi.org/10.18782/2320-7051.2315
Influence of Geographical Location on the Antioxidant Activity of Green Tea
Rasha Saad1 , Fadli Asmani2, Poornima3, Jiyauddin Khan2, Mohammed Kaleemullah2, Samer Al-dahlli2, Hamid Kazi2 and Brian Teo2
1College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences Ministry of National Guard
Health Affairs, Riyadh, Kingdom of Saudi Arabia
2School of Pharmacy, Management and Science University, Shah Alam 40100, Malaysia
3Biomedical Science , Faculty of Health & Life Sciences, Management and Science University,
Shah Alam 40100, Malaysia
*Corresponding Author E-mail: rshoo70@yahoo.com
Received: 15.06.2016 | Revised: 26.06.2016 | Accepted: 28.06.2016
Abstract
Antioxidants are man-made or natural substances that may prevent or delay some types of cell damage. This study was conducted in order to determine the impact of the geographical location on the antioxidant activity of five samples of green tea. The objective of this study was to extract the green tea, test the antioxidant activity of green tea extracts using, to screen the phytochemical elements using green tea extracts, as well as to study the chromatographical analysis based on the sampling location. Five green tea from different countries was obtained from Malaysia, China, Japan, London, and Australia. Then the green tea extracts was obtained by evaporation using rotary evaporator. The phytochemical elements were tested for saponin, flavonoid, alkaloid, tannin and phenol . The phytochemical screening had shown positive for all the elements tested. The antioxidant activity was done using 1, 1 –Diphenyl-2-picrylhydrazyl (DPPH) reagent which is a stable free radical. The antioxidant activity shown that the China green tea has the best scavenging activity of 97% while the scavenging activity of other green teas respectively are Malaysia green tea is 49% , Japan green tea has 77% , London green tea has 50% and Australia green tea has 70%. Finally, the thin- layer chromatography analysis revealed the compounds of flavonoid, catechin and phenol. This study found that there is a relationship between the geographical location and the antioxidant activity of green tea.
Keywords: Antioxidants, cell damage, geographical location, antioxidant activity, five green tea, DPPH, scavenging activity , thin-layer chromatography , flavonoid , catechin , phenol.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Saad1 , R., Asmani, F., Poornima, Khan, J., Kaleemullah, M., Al-dahlli, S., Kazi, H. and Teo, B., Influence of Geographical Location on the Antioxidant Activity of Green Tea, Int. J. Pure App. Biosci.4(3): 56-63 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2315
