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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (45) Last page : (55)
Article doi: http://dx.doi.org/10.18782/2320-7051.2302

Effects of adding cladode and epidermis extracts of Opuntia ficus-indica and Opuntia atropes to aerobic mesophilic bacteria and total coliforms in bovine raw milk

Lauro Antonio Delgado1, Rosa Elena Pérez1*, Rafael Roman2, Pedro Antonio Garcia1, Juvenal Esquivel1, and Ruy Ortiz2
1Facultad de Agrobiología “Presidente Juárez”- Universidad Michoacana de San Nicolás de Hidalgo. Paseo Lázaro Cárdenas-Berlín Col. Viveros. Uruapan Michoacán, México; 2Facultad de Ciencias Veterinarias. Universidad del Zulia. Maracaibo, Venezuela. 3Facultad de Medicina Veterinaria y Zootecnia-Universidad Michoacana de San Nicolás de Hidalgo. Km 9,5 Carretera Morelia-Zinapécuaro. Tarímbaro Michoacán, México. CP 58880
*Corresponding Author E-mail: rosa_elenap@yahoo.com
Received: 3.06.2016  |  Revised: 17.06.2016   |  Accepted: 22.06.2016  

Abstract
The aim of this research was to evaluate the effect of methanolic extracts from cladodes and cladode epidermis of Opuntia ficus-indica and Opuntia atropes on bovine raw milk microbiology. Methanolic extracts were obtained by maceration and added to bovine raw milk. Five repetitions per treatment were performed. A microbiological analysis was carried out 2 h after methanolic extracts were added. Aerobic mesophilic bacteria and total coliforms CFU mL-1 in raw milk decreased (p < 0.05) when Opuntia ficus-indica and Opuntia atropes extracts were added (≤ 3.1x105 and 1.5x106, respectively for aerobic mesophilic bacteria) and (1.9x105 and 1.2 x106, respectively for  total coliforms). Adding this methanolic extracts to raw milk achieved Mexican quality standards for aerobic mesophilic bacteria; but not for total coliforms. However, both species reduced aerobic mesophilic bacteria and total coliforms CFU mL-1 counts improving microbiological quality of bovine raw milk.

Key words: quality of milk, Opuntia spp., secondary metabolites, polar extracts.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782


Cite this article: Delgado, L.A., Pérez, R.E., Roman, R., Garcia, P.A., Esquivel, J. and Ortiz, R., Effects of adding cladode and epidermis extracts of Opuntia ficus-indica and Opuntia atropes to aerobic mesophilic bacteria and total coliforms in bovine raw milk, Int. J. Pure App. Biosci.4(3): 45-55 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2302