International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (16) Last page : (20)
Article doi: http://dx.doi.org/10.18782/2320-7051.2293
Embryo-Toxicity and Teratogenicity of Derris elliptica Leaf Extract on Zebra Fish (Danio rerio) Embryos
Josephine Joy V. Tolentino1* and Jerwin R. Undan2
1Graduate Student, Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz, Nueva Ecija, 3120 Philippines
2Molecular Biology and Biotechnology Laboratory, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz, Nueva Ecija, 3120 Philippines
*Corresponding Author E-mail: jjvtolentino0126@gmail.com
Received: 19.05.2016 | Revised: 2.06.2016 | Accepted: 7.06.2016
Abstract
The toxic effects of Derris elipticaor “Opay” were assessed against zebrafish embryos. Leaf extract of D. elliptica via hot water extraction and the zebra fish (Danio rerio) as the model organism were utilized for the assessment of its teratogenicity and embryo-toxicity potential. Two concentrations of 0.05% (T2) and 0.50% (T3) of leaf extract were used, while embryo water medium was used as the control (T1). Percent mortality was observed after 12, 24, and 48 hours. In addition, percent hatchability was observed after 48 hours only. The number of heart beats per minute of the zebra fish embryo within 48 hours post treatment was also recorded. The results showed that zebra fish embryos treated with 0.05% extract showed reduced hatchability rate, lower heartbeat rate and delayed formation compared with the control. The adverse effect of 0.50% treatment was still severe resulting in undeveloped head and tail region, coagulation and death of embryos. It was concluded that teratogenic and lethal effects on zebra fish increased with the increase in the dose concentration of leaf extracts of D. elliptica. Further investigation on this plant species affecting other animals with ethanol and acetone extracts are also recommended.
Key words: Teratogenic, Embryo, Zebra fish, Derris elliptica, Hot water leaf extract
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Tolentino, J.J.V. and Undan, J.R., Embryo-Toxicity and Teratogenicity of Derris elliptica Leaf Extract on Zebra Fish (Danio rerio) Embryos, Int. J. Pure App. Biosci.4(3): 16-20 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2293
