International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (201) Last page : (205)
Article doi: http://dx.doi.org/10.18782/2320-7051.2318

Multiple Shoot and Aerial Roots Induced from Various Explants of

P. tetragonolobus L. (Winged Bean)

D.S.R. Naik*, T.N. Swamy  and A. Seetaram Naik
Department of Botany, Kakatiya University, Warangal, Telangana State, India
*Corresponding Author E-mail: ravinaik0805@gmail.com
Received: 16.06.2016  |  Revised: 23.06.2016   |  Accepted: 27.06.2016  

Abstract
Multiple shoots and aerial roots were directly regenerated in leaf and stem explants cultured on MS+BAP+IAA, BAP+NAA alone or in combination. MS+BAP (2.0 mg/L)+IAA (0.5 mg/L) respectively induced maximum number of multiple shoots with green callus, after four weeks of culture. This culture was transferred on ½ MS medium supplemented with BAP (2.0 mg/L)+IAA (0.5 mg/L) produced maximum number of multiple shoots and aerial roots from leaf explants, after eight weeks of culture. MS media supplemented with BAP (2.0 mg/L)+NAA (0.5 mg/L), induced adventitious roots with callus. These cultures were transfer on the media produced tuberous roots.

Key words:Winged Bean, Explants, Leaf, Stem

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Naik, D.S.R., Swamy, T.N.  and Seetaram Naik, A., Multiple Shoot and Aerial Roots Induced from Various Explants of P. tetragonolobus L.(Winged Bean), Int. J. Pure App. Biosci.4(3): 201-205 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2318