International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (160) Last page : (166)
Article doi: http://dx.doi.org/10.18782/2320-7051.2311
Studies on Molecular Variability Among Xanthomonas axonopodis pv. punicae Isolates Collected from Different Locations
Swaranjali Kishor Gadhe1*, Suresh Haribhau Antre2, Bhausaheb Babanrao Ghorpade3,
Rishikesh Haribhau Autade4 and Rushil Ramesh Mandlik5
1,2Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, ITI Campus, Chandrapur Road, Tal. - Rahata, Dist- Ahmednagar 413736, (M.S.), India
3,4,5Department of Plant Biotechnology, College of Agricultural Biotechnology, Loni, ITI Campus, Chandrapur Road, Tal. - Rahata, Dist- Ahmednagar 413736, (M.S.), India
*Corresponding Author E-mail: gadheswaranjali@gmail.com
Received: 7.06.2016 | Revised: 15.06.2016 | Accepted: 19.06.2016
Abstract
Bacterial blight is one of the most important disease of Pomegranate caused by bacterium Xanthomonas axonopodis pv. punicae (Xap). The disease is characterized by small, irregular, translucent, water soaked spots with light to dark necrotic centre surrounded by prominent yellow margin on leaves. The isolates were made from the symptomatic samples collected from five different locations viz. Loni, Astagaon, Talegaon, Sangamner and Rahuri belonging to Ahmednagar district of Maharashtra state, India.
The variability was studied among the five isolates of Xanthomonas axonopodis pv. punicae by using 10 RAPD primers, out of which 07 primers produces total 26 scorable bands with an average of 3.7 bands per primer. Out of 26 bands, 21 bands were found to be polymorphic and level of polymorphism was 80.76%. The Jaccard’s similarity coefficient showed that the isolate Loni (Code Xap I) was found to have higher value of similarity coefficient 0.88 with Astagaon (Code Xap II), whereas Talegaon (Code Xap III) was found to have lower value of similarity coefficient 0.20 with Rahuri (Code Xap V). The cluster analysis based on similarity coefficients divides the five isolates into two major and two sub clusters. These results indicate a high level of genomic variability among the isolates even within the same geographical regions.
Key words :Bacterial blight, Pomegranate, polymorphism, RAPD, Xanthomonas axonopodis pv. punicae.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Gadhe, S.K., Antre, S.H., Ghorpade, B.B., Autade, R.H. and Mandlik, R.R., Studies on Molecular Variability Among Xanthomonas axonopodis pv. punicae Isolates Collected from Different Locations, Int. J. Pure App. Biosci.4(3): 160-166 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2311
