International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (154) Last page : (159)
Article doi: http://dx.doi.org/10.18782/2320-7051.2301

The Frequency of Pseudomonas aeruginosa Clinical isolates in a Tertiary Care Hospital

M. Anitha*, D.M. Monisha, A. Mohamed Sulthan, K. Pratikshia and S.R. Swathy
Department of Microbiology, Shri Sathya Sai Medical College & Research Institute, Thiruporur, Sri Balaji Vidyapeeth University, Tamil Nadu, India
*Corresponding Author E-mail: animalar03@gmail.com
Received: 2.06.2016  |  Revised: 14.06.2016   |  Accepted: 17.06.2016  

Abstract
Background:
Pseudomonas aeruginosa is a nosocomial pathogen distributed worldwide.This study examined the antimicrobial resistance patterns of P. aeruginosa clinical isolates procured from hospitalized patients.
Aims and objectives:
To study the Resistance pattern of Pseudomonas aeruginosa isolates from miscellaneous samples.
Materials and methods:
A total number of 125 Pseudomonas strains were isolated from 904 miscellaneous samples (Sputum, HVS, Pus, Ear swab) between January 2016 to March  2016. Samples were obtained from the hospitals and processed in our central Microbiology lab. Antimicrobial susceptibility test were performed by Kirby Bauer disc diffusion method as per the recommendations of  NCCLS guidelines.
Result:
P. aeruginosa were isolated from125 miscellaneous samples such as sputum (49%) followed by HVS (27%),  pus and wound swab (22%) & ear swab(2%). Most effective antibiotic was the Aminoglycosides drugs– Amikacin (93.6%), Gentamicin(76%), followed by Carbapenem drug- Imipenem (80%) and Fluoroquinolones drug-Ciprofloxacin (72.8%).Our results reveal high resistance to Penicillin drug- Amoxicillin (95.2%) and  1st, 3rd generation Cephalosporins (Cefazoline (92.8%), Cefotaxime (88.8%)).
Conclusion:
This study implicates that, the organism isolated from various clinical specimens were highly sensitive to Amikacin, which is followed by Imipenem, Gentamicin, Ciprofloxacin. In hospitalized patients, the emergence and spread of the resistance can be reduced by regular monitoring of drug resistance and  strict  infection control measures should be ensured.

Key words: Antimicrobial resistance, Clinical isolates, Pseudomonas aeruginosa

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Anitha, M., Monisha, D.M., Sulthan, A.M., Pratikshia, K.  and Swathy, S.R., The Frequency of Pseudomonas aeruginosa Clinical isolates in a Tertiary Care Hospital, Int. J. Pure App. Biosci.4(3): 154-159 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2301