International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (136) Last page : (143)
Article doi: http://dx.doi.org/10.18782/2320-7051.2291

Enzymatic Responses to Pesticide Chlorpyrifos Exposures in Kidney of Fish Gambusia affinis

Neelam Sharma¹*, Sudha Summarwar² and Jyoutsna Pandey^3
1Department of Zoology, Agrawal Girls College, Gangapur City, (S.W.M.) Rajasthan, India
^2,3Department of Zoology, S.D. Government College, Beawar 
*Corresponding Author E-mail: drsharmaneelam1@gmail.com
Received: 17.05.2016  |  Revised: 29.05.2016   |  Accepted: 5.06.2016  

Abstract
The present study was conduct to assess the effect of Chlorpyrifos toxicity to enzymatic response to fish Gambusia affinis. Alterations in certain enzymes have been examined in the kidney of a fish, after exposure to 1/10th, 1/3rd and sub-lethal (LC50) dose of chlorpyrifos. Untreated fish served as control. Enzyme estimations of ACP, ALP, LDH and ATPase activity in kidney of control as well as in test Gambusia affinis were done.
The enzyme activity ACP, ALP and LDH was increased throughout the experimental period while the ATPase activity was decline along the experimental period at three different concentrations of chlorpyrifos was observed. Enzymatic studies are good parameters which help to see the effect of pesticide on biochemical composition of vital tissue of fish. Hence attempt has been made to find out enzymatic response in tissue like kidney of fish.

Keywords: Chlorpyrifos, Gambusia affinis, Kidney, LC50.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sharma, N., Summarwar, S. and Pandey, J., Enzymatic Responses to Pesticide Chlorpyrifos Exposures in Kidney of Fish Gambusia affinis, Int. J. Pure App. Biosci.4(3): 136-143 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2291