International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (130) Last page : (135)
Article doi: http://dx.doi.org/10.18782/2320-7051.2304
Isolation and Purification of Cuticle Degrading Extra Cellular Proteases from Entomopathogenic Fungal Species of Beauveria bassiana
A.G. Vikhe1*, N.S. Dale2, R.B. Umbarkar3, G.B. Labade4, A.R. Savant5, and A.A. Walunj6
1Research Scholar, 2Associate Professor of Entomology, 3Associate Professor of Agronomy,
4Assistant Professor of Botany, 5Assistant Professor of Plant Biotechnology,
6Assistant Professor of Plant Pathology and Microbiology
Department of Agril. Entomology, College of Agriculture and Agril. Biotechnology, LONI-413736
Tal- Rahata, Dist-Ahmednagar, Maharashtra India
*Corresponding Author E-mail: akshay.vikhe99@gmail.com
Received: 5.06.2016 | Revised: 16.06.2016 | Accepted: 18.06.2016
Abstract
The entomotoxic protein of Beauveria bassiana (Bals.) (Ascomycota:Hypocreales) was precipitated with 90% saturated (NH4)2SO4 and the precipitate was eluted in Sephadex G-25 column. The protein content was higher (0.026 μg/ml) supported by UV spectroscopy absorbance. The vibration frequency in FT-IR appeared between the ranges of 1650– 1580cm-1 due to N-H bond formation for amines. Bioassays of Beauveria bassiana reveal that caused significantly higher insecticidal activity.Amylase, Protease and Lipase (digestive enzymes), Esterase, Glutathione S-Tranferase and Lactate dehydrogenase level/activity in insects. Hence, the Beauveria bassiana was subjected to sodium dedosyl sulphate–poly-acrylamide gel electrophoresis (SDS-PAGE)
Key word: Beauveria bassiana, Protein Extraction, FT-IR, SDS-PAGE
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Vikhe, A.G., Dale, N.S., Umbarkar, R.B., Labade, G.B., Savant, A.R. and Walunj, A.A., Isolation and Purification of Cuticle Degrading Extra Cellular Proteases from Entomopathogenic Fungal Species of Beauveria bassiana, Int. J. Pure App. Biosci.4(3): 130-135 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2304
