International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (104) Last page : (111)
Article doi: http://dx.doi.org/10.18782/2320-7051.2312

Analysis of Fish Oil as Potential Oxidative Stress Inhibitor in C57BL/6 Mice

Samina Bashir, Yadhu Sharma and Farah Khan*
Department of Biochemistry, Hamdard University, New Delhi, INDIA
*Corresponding Author E-mail: farahkhan3@gmail.com
Received: 10.06.2016  |  Revised: 19.06.2016   |  Accepted: 24.06.2016  

Abstract
The aim of present study was to evaluate the effect of dietary supplementation of fish oil (FSO) on the oxidative stress of liver tissue in c57bl/6 mice. The thiobarbituric acid reactive substances (TBARS), xanthine oxidase (XO), superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-PX) were studied in mice after oral gavage of 4, 8 or 16 mg/ kg body weight (bw.) FSO (rich in omega-3 fatty acids, eicosapentaenoic acid and docosahexaenoic acid) for 4 weeks and the levels were compared to untreated control mice. The mice in the treated group had significantly higher SOD activity (P<0.01), GSH (P<0.01), catalase (P<0.01) and decreased TBARS levels (P< 0.01) with respect to control mice whereas XO activities were not observed to be significantly different between the groups. FSO supplementation also caused a significant (p < 0.05) decrease in inflammatory cytokines (TNF-α, and IL-1β) and increase in the anti-inflammatory ones (IL-10) at a dosage of 16 mg/kg bw.
Thus, it can be concluded that dietary supplementation with FSO may enhance resistance to oxidative stress and validate the notion that FSO is an effective dietary supplement for management of various oxidative stress induced diseases.

Keywords: Catalase, fish oil, Glutathione reductase, polyunsaturated fatty acids, reactive oxygen species, Superoxide dismutase.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bashir, S., Sharma, Y. and Khan, F., Analysis of Fish Oil as Potential Oxidative Stress Inhibitor in C57BL/6 Mice, Int. J. Pure App. Biosci.4(3): 104-111 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2312