International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (73) Last page : (79)
Article doi: http://dx.doi.org/10.18782/2320-7051.2317

Acute Oral Rat Toxicity Study of Multi-herbal formulation (ECD0058)

Rohan Thomas* and P. G. Kale
Department of Zoology, Hindi Vidya Prachar Samiti's, Ramniranjan Jhunjhunwala College, Ghatkopar (West), Mumbai
*Corresponding Author E-mail: rohanthomas83@yahoo.com
Received: 20.06.2016  |  Revised: 28.06.2016   |  Accepted: 30.06.2016  

Abstract
Background and objective: The current study was designed to study acute oral toxicity study of multi-herbal formulation ECD0058 (M/S Enki Lifescience Limited, Mumbai, India) according to OECD guidelines. For new substances it is the recommended stepwise testing approach for developing scientifically sound data on the safety of the substance.
Conclusions: 12 female Wistar rats were selected for acute oral toxicity study of ECD0058. The animals were fasted for minimum 16 hours prior to dosing and for 4 hours post dosing, feed was withheld but drinking water provided ad libitum. 3 rats of the first group G1 were dosed with starting dose of 300 mg/kg body weight and the animals did not show any mortality. So another 3 animals of the same group G1 were dosed with 300 mg/kg body weight and no mortality was observed. Next, 3 animals of group G2 were dosed with 2000 mg/kg body weight and no mortality was observed. So, another 3 animals of the same group G2 were dosed with 2000 mg/kg body weight and no mortality was observed. Hence, further dosing was stopped.
At 300 and 2000 mg/kg body weight, all the animals were observed normal throughout the experimental period.

Key words: Enki Lifescience Limited, Toxicity, Rat

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Thomas, R. and Kale, P.G., Acute Oral Rat Toxicity Study of Multi-herbal formulation (ECD0058), Int. J. Pure App. Biosci.4(3): 73-79 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2317