International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (296) Last page : (303)
Article doi: http://dx.doi.org/10.18782/2320-7051.2252

Humoral Immunity studies of Fresh Mud Crab Scylla Serrata Forsskal, 1775

CH. Saritha Subhashini. and P. Nagarajarao*
Department of Zoology, Osmania University, Hyderabad – 500 020, India
*Corresponding Author E-mail: nagarajaraop@yahoo.com
Received: 27.03.2016  |  Revised: 12.04.2016   |  Accepted: 14.04.2016  

Abstract
Phenoloxidase is a key enzyme in the innate immunity of the crab. It protects the animal from injury, infection, hardening of exoskeleton and melanisation. Whereas Lysozyme is a bacteriolytic enzyme secreted by host during phagocytosis. It is one of the important enzymes in the innate immunity of the crab against gram positive and gram negative bacteria.  In this study we have studied that Phenoloxidase activity increased from 2hrs to 48 hrs after challenge with  gram positive and gram negative bacteria in case of male crabs. In case of female crabs challenged with bacteria, phenoloxidase activity gradually increased from 2hrs to 48 hrs of post bacterial inoculation. The results of the present study revealed that the enzyme activity in hemolymph of bacterial challenged crabs was highest in all time points than control and control injured crabs. The zone of inhibition increase gradually from 2hrs to 12 hrs in Klebsiella challenged male crab and female crab and also in male and female crabs challenged with Bacillus. The protein band observed at 16 kDa was identified as Lysozyme and 67kDa as Prophenoloxidase.

Key words: Prophenoloxidase, lysozyme, antimicrobial studies, Scylla Serrata

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Subhashini, S. and Nagarajarao, P., Humoral Immunity studies of Fresh Mud Crab Scylla Serrata Forsskal, 1775, Int. J. Pure App. Biosci.4(2): 296-303 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2252