International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (256) Last page : (262)
Article doi: http://dx.doi.org/10.18782/2320-7051.2257

Effect of Cellulase Production by Fungi Cultured on Banana Waste

S. Madhavan¹ and R. Mangalanayaki²*
¹PG and Research Department of Botany, Rajagopalasamy Art College,
Mannargudi, Thiruvarur District, Tamilnadu - 614 001
²PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Sundarakkottai, Mannargudi, Thiruvarur District, Tamilnadu - 614 016
*Corresponding Author E-mail: sivamangalamsree@gmail.com
Received: 31.03.2016  |  Revised: 11.04.2016   |  Accepted: 14.04.2016  

Abstract
Cellulase production from cellulosic banana waste using Trichoderma harzianum, Aspergillus niger and Fusarium oxysporum was assessed. The wastes were dried, pre-treated with alkali and steam, re-dried and then blended. The powdered wastes were then used as substrates in separate shake-flasks which contained mineral salts medium (MSM) and inoculums of Trichoderma harzianum, Aspergillus niger and Fusarium oxysporum. Fermentations were carried out in flasks containing the MSM, the waste substrate and the inoculum at pH 5.0, 1% substrate concentration and cultured on a rotary shaker at 29±1ºC initially for 5 days to verify cellulase production by the organisms from the waste substrates, then for 7 days or 9 days while varying different fermentation parameters. Cellulase activity and amount of glucose produced by the three test organisms from the waste substrates were determined and compared. The amount of glucose produced was optimized by varying the fermentation parameters: Time, pH, Substrate concentration and Temperature. The results obtained from the fermentations showed that Trichoderma harzianum, produced the highest amount of glucose among the cultures tested. This was produced from banana pulp at pH 4 and temperature of 45ºC on Day 7 of fermentation. The highest amount of glucose produced by Aspergillus niger was also from banana pulp at pH 3 and temperature of 40ºC on Day 5 of fermentation. The highest amount of glucose produced by Fusarium oxysporum was from banana pulp (0.54mg/0.5ml) at pH 4 and temperature of 45ºC on Day 5 of fermentation.

Key words: Banana peel, Banana pulp, Cellulase and waste

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Madhavan, S. and Mangalanayaki, R., Effect of Cellulase Production by Fungi Cultured on Banana Waste, Int. J. Pure App. Biosci. 4(2): 256-262 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2257