International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (211) Last page : (217)
Article doi: http://dx.doi.org/10.18782/2320-7051.2273
Antifungal activity and investigation of bioactive compounds of marine intertidal bivalve Gafrarium divaricatum from West coast of India
Atul G. Babar1,3*, Anant Pande1,2,3 and Balasaheb G. Kulkarni1,3
1Department of Zoology, The Institute of Science, 15 Madame Cama Road, Mumbai- 400032, Maharashtra, India
2Wildlife Institute of India, Chandrabani, Dehradun-248001, Uttarakhand, India
3Society for Health of Ocean Resources and Environment, Vasind - 421601, District Thane, Maharashtra, India
*Corresponding Author E-mail: babaratul_g@yahoo.co.in
Received: 20.04.2016 | Revised: 27.04.2016 | Accepted: 29.04.2016
Abstract
Crude methanolic extract of Gafrarium divaricatum and its five fractions viz. hexane, ethyl acetate, chloroform, acetone and water were screened against four fungal human pathogens to determine antifungal activity by using disc-diffusion assay method. Only crude methanolic extract showed consistent antimicrobial activity as compared to other fractions. Crude methanolic extract showed significant antifungal activity against Cryptococcus neoformans, Aspergillus flavus and Aspergillus niger with Minimum Inhibitory Concentration 0.1 mg/ml, 0.3 mg/ml and 0.3 mg/ml respectively. Determination of the possible chemical antifungal components from crude methanolic extract was carried out by GC-MS Technique. This analysis identified and characterized nine bioactive compounds. Four of these compounds that have known therapeutic properties include (i) Octadecane, 2,2,4,15,17,17-hexamethyl-7,12-bis(3,5,5-trimethylhexyl) for anticancer properties, (ii) l-(+)-Ascorbic acid 2,6-dihexadecanoate for antitumour and antibacterial properties and (iii) 2-Cyclopentene-1-tridecanoic acid for antibacterial activity (iv)17-(1,5-Dimethylhexyl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol as drug for mineral disorders and antibacterial properties. In this study, G. divaricatum was found to be a potential source of medicinal compounds.The five other compounds obtained in the present study should be screened for their medicinal properties.
Key word: Antifungal; Bivalve; GC-MS analysis; Minimum inhibitory Concentration; Mumbai coast.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Babar, A.G., Pande, A. and Kulkarni, B.G., Antifungal activity and investigation of bioactive compounds of marine intertidal bivalve Gafrarium divaricatum from West coast of India, Int. J. Pure App. Biosci.4(2): 211-217 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2273
