International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (177) Last page : (182)
Article doi: http://dx.doi.org/10.18782/2320-7051.2233

Determination of Minimum Inhibitory Concentration of organic extract of Catharanthus roseus by a novel modified well diffusion technique

Apurv Gaur*, Malarvili Ganeshan1, Rameez Shah3 and A.D. Bholay4
*Department of Microbiology, K.S.K.W. A.S.C. College, Savitribai Phule Pune University, Nashik, M.H., India
1NADIR Godrej Centre for animal research and development, Peth Road, Dindori, Nashik-422003
3PG Department of Microbiology, K.T.H.M. College, Savitribai Phule Pune University, Nashik, M.H., India
4Department of Health Care Administration, Maharashtra University of Health Sciences, Nashik, M.H., India
*Corresponding Author E-mail: gaur.apurv@gmail.com
Received: 4.03.2016  |  Revised: 12.03.2016   |  Accepted: 15.03.2016  

Abstract
India has a tremendous biodiversity. It has optimum conditions for a number of medicinal plants that thrive naturally here. Catharanthus roseus also known as Sadabahar or Sadhaphuli by vernacular names is one among many medicinal plants commonly occurring here. C. roseus has always remained in limelight for its various anticancerous properties imparted by various alkaloids. In this research paper, a new microbial susceptibility test method is described, which retains the simplicity of Epsilometer test for determination of MIC. In this method, organisms can be tested directly for susceptibility to known concentration of plant extracts and antibiotics in agar well. Ranges of concentrations are used to determine the minimum inhibitory concentration. Using dried leaves of C. roseus, organic extracts were prepared in acetone and ethyl acetate solvent using soxhlet apparatus. MIC values of extracts were determined by E-test method against pathogens like Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. MIC values evaluated were 25µg/ml and 12.5µg/ml for E.coli, 12.5µg/ml for P. vulgaris and 6.25µg/ml and 50µg/ml for K. pneumoniae in acetone and ethyl acetate extracts respectively. The MIC values obtained by E Test are lower than those reported in literature using broth dilution method. Modified well diffusion method is efficient, less time consuming and simpler. C. roseus has a great potential as an antimicrobial agent, which needs to be explored further intensively.

Keywords: Catharanthus roseus, Modified well diffusion, MIC, Acetone, Ethyl acetate

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Gaur, A., Ganeshan, M., Shah, R. and Bholay, A.D., Determination of Minimum Inhibitory Concentration of organic extract of Catharanthus roseus by a novel modified well diffusion technique, Int. J. Pure App. Biosci. 4(2): 177-182 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2233