International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (103) Last page : (110)
Article doi: http://dx.doi.org/10.18782/2320-7051.2254
A Study on Inhibitory Effect of Trichoderma sp. TKD on Aspergillus flavus FNCC6109 and Its Molecular Identification
IBG Darmayasa1* and I Gst Lanang Oka2
Microbiology Laboratory (FMIPA) Faculty of Mathematics and Natural Sciences, Udayana University, Indonesia
*Corresponding Author E-mail: darm_aponk@yahoo.co.id
Received: 29.03.2016 | Revised: 11.04.2016 | Accepted: 15.04.2016
Abstract
Aspergillus flavus is one type of fungus that often cause problems in the field of agriculture and animal husbandry. Biological control is necessary in order to reduce the use of synthetic fungicides which have good side effect on humans and the environment. Exploratory research that has been done shows a candidate isolate potential to inhibit the growth of Aspergillus flavus. These isolates, isolated from the rhizosphere of corn and is named Trichoderma sp. TKD. The purpose of this study was to determine the species of Trichoderma sp. TKD and its ability in inhibiting the A. flavus FNCC6109. Identification of Trichoderma sp. TKD was done macroscopically, microscopically and molecularly using internal transcribed spacer region (ITS 5 F: 5 '-¬GAAGTAAAAGTCGTAACAAGG-- 3' and ITS 4 R: 5 '- TCCTCCGCTTATTGATATGC - 3'). The test of the inhibitory power used dual culture method. The results showed Trichoderma sp. TKD isolated from the rhizosphere of corn was identified as Trichoderma asperellum and had the potential to inhibit the growth of A. flavus FNCC6109 at 98.849±1.100%.
Keywords: Aspergillus flavus FNCC6109, Trichoderma sp.TKD, Aflatoxin
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Darmayasa, I.B.G. and Oka, I.G.L., A Study on Inhibitory Effect of Trichoderma sp. TKD on Aspergillus flavus FNCC6109 and Its Molecular Identification, Int. J. Pure App. Biosci. 4(2): 103-110 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2254
