International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (68) Last page : (74)
Article doi: http://dx.doi.org/10.18782/2320-7051.2206
3D Structure Prediction and Validation of Novel Exo-beta-1, 3-Glucanase from Psychroflexus torquis ATCC700755/ACAM623
Richard Terkuma Isa1,2*, Mariya Tijjani1, Malaz Khansa1,3, Sepideh Parvizpour1 and Mohd Shahir Shamsir1
1Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai Malaysia
2Dept. of Biological Sciences, Faculty of Pure and Applied Sciences, Federal University Wukari, 670001 Wukari Nigeria
3Dept. of Environmental studies, Higher Institute for Applied Science and Technology, P.O.Box 31983, Damascus Syria
*Corresponding Author E-mail: isa@fuwukari.edu.ng
Received: 31.01.2016 | Revised: 11.02.2016 | Accepted: 14.02.2016
Abstract
Exo-beta-1,3-glucanase (EC 3.2.1.58) successively hydrolyse O-glycosidic linkage of beta-1,3-linked glucans from their nonreducing terminus to subsequently yield glucose. In this paper, we present the 3D structure prediction and validation of novel bacterial exo-beta-1, 3-glucanase of GH family 17 from Psychroflexus torquis ATCC700755/ACAM623-a psychrophilic bacteria. This is the first attempt to model this enzyme with a GH family 17 fold in bacteria. All known exo-beta-1, 3-glucanase of GH family 17 are of plant and fungal origin. Validation of the stereochemistry of the predicted model using the Rampage sever for Ramachandran plot indicates 97.1% of residues located in favoured and allowed regions while the remaining are in outlier region. This model also obtained an ERRAT score of 93.17% which is well above the 50% critical value for a good model. As a way of further evaluation, we generated profile files of the model and template by the ‘asses_dope’ command of MODELLER 9.15 programme. These profile files were used in gnuplot 4.6 ploting software to produce a comparative plot of Discrete Optimized Protein Energy (DOPE) per residue of the template and model. This model obtained an overall DOPE value better than the template.
Key words: Psychroflexus torques, O-glycosidic, Exo-beta-1,3-glucanase.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Isa, R.T., Tijjani, M., Khansa, M., Parvizpour, S., Shamsir, M.S., 3D Structure Prediction and Validation of Novel Exo-beta-1, 3-Glucanase from Psychroflexus torquisATCC700755/ACAM623, Int. J. Pure App. Biosci. 4(1): 68-74 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2206
