International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (291) Last page : (295)
Article doi: http://dx.doi.org/10.18782/2320-7051.2229

Microprapogation of callus from Syzygium cumini explant

S.H. Jejurker, U.R. Tanpure, S.D. Pawar, R.R. Joshi and M.S. Wadikar*
Department of Biotechnology, Vinayakrao Patil College Vaijapur, Dist. Aurangabad (M.S) 423701 India
*Corresponding Author E-mail: mswadikar@yahoo.com
Received: 18.02.2016 | Revised: 25.02.2016 | Accepted: 27.02.2016
Abstract
Microprapogation of the syzygium cumini explant was tried by using the different kind of MS. medium, hormonal changes in the medium show variations in the result. At the appropriate quantity of the hormones the explant shows a significant amount of development; the basal medium does show the some kind of development into a bud & no effect on the leaf explant development. The auxin is generally root initiator and cytokinin are the shoot developers. Very low level of growth regulator concentration does not show the significant result. (0.1-mg/l -0.1 mg/l) Present work we got development of callus by using a MS medium of basal medium + 6 BAP with additional supplement of IBA and kinetin at concentration (0.60 mg/l – 0.60 mg/l) and at the (0.02-0.5 mg/l) of IBA & kinetin shoot development observed. IAA only shows some growth in explant but does not develop the tumor/ callus.

Key words: Syzygium Cumini, Explant, Basal Medium, Callus, Growth Hormones

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Jejurker, S.H., Tanpure, U.R., Pawar, S.D., Joshi, R.R. and Wadikar, M.S., Micropropogation of callus from Syzygium cumini explant, Int. J. Pure App. Biosci. 4(1): 291-295 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2229