International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (273) Last page : (277)
Article doi: http://dx.doi.org/10.18782/2320-7051.2224

Extraction of Collagen Protein by Acid Soluble Collagen from Soya Seeds

Komal, Anu Kumar*, Mamta, Deepak Kala, Sunny Dhiman and Tulika Mishra
University Institute of Biotechnology, Chandigarh University, Mohali Punjab, INDIA
*Corresponding Author E-mail: anubiotech88cu@gmail.com
Received: 18.02.2016 | Revised: 25.02.2016 | Accepted: 27.02.2016
Abstract
Considering the two collagen rich foods and extracted out the collagen protein from it by the method of acid soluble collagen (ASC). Collagen is naturally occurring fibrous protein which is important in maintaining the health and vitality of connective tissues. They work with elastin, another structural protein to maintain connective tissues elasticity. They may get benefit from the two amino acids lysine and proline and the presence of collagen promoting nutrient such as vitamin C. In the present study determination was done by ASC in which the sample was dissolved in 30 ml of 0.1N NaOH and filtration was done. After that the supernatant took as acid soluble followed by precipitation with 2.6M NaCl involving centrifugation at 38,400rpm for about 20 minutes and the residues were separated. At last, wet and dried separated residues were taken for protein determination by kjeldahl’s method. From this study it was concluded that the dry and wet percentage of protein is more than the total soy protein which means that the extracted protein was collagen.

Key words: Acid soluble, Collagen, Protein, Sources.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Komal, Kumar, A., Mamta, Kala, D., Dhiman, S. and Mishra, T., Extraction of Collagen Protein by Acid Soluble Collagen from Soya Seeds, Int. J. Pure App. Biosci. 4(1): 273-277 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2224