International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (216) Last page : (225)
Article doi: http://dx.doi.org/10.18782/2320-7051.2210

Effect of herbicide Butachlor on the earthworm Eutyphoeus waltoni Michaelsen

Vandana Singh and Keshav Singh*
Vermibiotechnology Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, 273 009, UP, India
*Corresponding Author E-mail: keshav26singh@rediffmail.com
Received: 4.02.2016 | Revised: 10.02.2016 | Accepted: 16.02.2016
Abstract
The lethal effect of herbicide butachlor (2-chloro 2,6diethyl N, butoxymethyl acetanilide) on the earthworm Eutyphoeus waltoni was evaluated in different combination of feed materials under the laboratory conditions. Eutyphoeus waltoni were exposed to different concentrations of butachlor (0.3, 0.6, 0.9, 1.2 mg/kg) in feed material i.e. buffalo dung, wheat straw and gram bran and soils for time range from 24 to 240h.The maximum toxicity was observed in the Sandy soil 24, 48, 72, 96, 120,  and 240h with LC50 values ( 95% of confidence limits) estimated by probit analysis were 0.952 (0.689 to 2.071), 0.652 (0.486 to 0.852), 0.543 (0.346 to 0.724), 0.449 (0.246 to 0.602), 0.388 (0.127 to 0.533) and 0.287 (0.056 to 0.432), respectively. There were dose and time dependent increase in the mortality rate due to exposure to the herbicide. Maximum toxicity was observed in the sandy soil whereas, minimum in combination of buffalo dung with gram bran.

Key words: Toxicity, Herbicides, Butachlor, Earthworm, Soil, Buffalo dung, Agro-wastes.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Singh, V. and Singh, K., Effect of herbicide Butachlor on the earthworm Eutyphoeus waltoniMichaelsen, Int. J. PureApp. Biosci. 4(1): 216-225 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2210