International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (172) Last page : (179)
Article doi: http://dx.doi.org/10.18782/2320-7051.2193
Protease Inhibitor Associated Antimicrobial Activity of Pea Pisum sativum L. cv. Arkel
Huma Habib1*, Khalid Majid Fazili2, Mohammad Afzal Zargar3 and Bashir Ahmed Ganie4
1Department of Biochemistry, Islamia College of Science and Commerce, Srinagar 190003, India
2Department of Biotechnology, University of Kashmir, Srinagar 190006, India
3Registrar, Central University, Srinagar, 190015, India
4Director CORD, University of Kashmir, Srinagar 190006, India
*Corresponding Author E-mail: huma99@gmail.com
Received: 17.01.2016 | Revised: 26.01.2016 | Accepted: 01.02.2016
Abstract
Aqueous and organic extracts were prepared from fresh pea seeds by standard methods. The extracts were analyzed for protease inhibitory activity using Dot,blot assay. The aqueous as well as the organic extracts obtained with ethanol showed significant protease inhibitory activity. The aqueous extracts were treated with ammonium sulfate to cause differential protein precipitation. 30%, 60% and 90% ammonium sulfate saturations were used to prepare these different protein fractions. 60% and 90% fractions were positive for protease inhibitor (PI) activity on dot blot assay, the 30% fraction did not show any inhibitory activity. The assay of antibacterial activity by disc diffusion method using E.coli (ATCC 25922) strain revealed that the crude aqueous extract, 90% fraction and ethanolic extract were highly active in inhibiting the bacterial growth, the 60% fraction showed moderate antibacterial activity and the 30% fraction was not significant.
Key words: Antibacterial activity, Protease inhibitor, Pisum sativum
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Habib, H., Fazili, K.M., Zargar, M.A. and Ganie, B.A., Protease Inhibitor Associated Antimicrobial Activity of Pea Pisum sativum L. cv. Arkel, Int. J. Pure App. Biosci. 4(1): 172-179 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2193
