International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (126) Last page : (132)
Article doi: http://dx.doi.org/10.18782/2320-7051.2188

Potential of Bacillus subtilis to produce acidic protease under mutagenic condition

Prasanth M., Nitin Marathe, Shubham Jadhav, Saharika Slathia, Ramankannan A., Shanthini T. and Ramesh N.*
School of Bio-Sciences and Technology, VIT University, Vellore, Tamil Nadu, India
*Corresponding Author E-mail: drpnramesh@gmail.com
Received: 13.01.2016 | Revised: 20.01.2016 | Accepted: 26.01.2016
Abstract
Protease from Bacillus subtilis has wide applications in industrial sectors.A total of 28 bacteria were isolated from marine mud sample, of which 3 isolates exhibitedconstructive results for protease production in skim milk, gelatin and casein agar. Among three isolates, MMK 2/8 had superlativeenzymatic activity in skim milk broth (170 U/mL) and was subjected for taxonomical identification, which was found to be Bacillus subtilis. The finest conditions were found to be at pH 6.0, temperature 40 oC and 1% NaCl concentration and their maximal enzyme activitywas 354 U/mL, 437 U/mL and 263U/mL respectively, at 36 hrs of incubation. The molecular weight of the protease enzyme was considered to be approximately 50 kDa by SDS-PAGE. The enzyme activity was enriched by exposing it to ultra violet radiation [UV] for 60secand it was observed to be 610 U/mL. Hence, we charted that the production of acidic protease treated with ultra violet radiation was maximum when compared with the parental strain.

Key words: Acidic protease, Bacillus subtilis, UV radiation, skim milk agar

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Prasanth, M., Marathe, N., Jadhav, S., Slathia, S., Ramankannan, A., Shanthini, T. and Ramesh, N., Potential of Bacillus subtilis to produce acidic protease under mutagenic condition, Int. J. Pure App. Biosci. 4(1): 126-132 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2188