International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2015, Volume : 3, Issue : 6
First page : (42) Last page : (58)
Article doi: http://dx.doi.org/10.18782/2320-7051.1891
The Study of Nanoparticle Size and Coating Influence on the Protein Corona Thermodynamic
Seyed Mohammad Motevallia,b and Fateme Mirzajania,b,*
aDepartment of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NET), Shahid Beheshti University, G.C. Evin, Tehran, Iran.
bDepartmrnt of Nanobiotechnology, Protein Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran
*Corresponding Author E-mail: f_mirzajani@scientist.com
Abstract
Nanoparticle-protein interaction is one of the important aspects of xenobiotic materials in biological environments. Larger nanoparticles can effect on proteins structure and caused more decrements in their hydrophilicity. All proteins have certain binding affinity to nanoparticles called, Vroman effect. This effect is coupled with free Gibbs energy alteration in energy profile of free proteins and their adsorbed on nanoparticles. However, there are no evidences that show how and why hard corona is a protein fingerprint. This study showed that, larger proteins having higher molecular weight and less helix structures attitude to larger nanoparticles. According to statistical analysis, unlike previous studies, it found that the size of nanoparticles is more important than their hydrophobicity. From a thermodynamic point of view, larger nanoparticles have more contact positions than the smaller; so whole energy transmitted from larger nanoparticles is higher. This result indicated that the energy transmitted from smaller nanoparticles to protein, could be transferred to aqueous environment more than larger ones. In addition it demonstrated that, energy parameter could be used as reference parameter for explaining protein attitude to nanoparticle. Whereas nanoparticles can play a role as seeds for protein fibrillation, it is important to consider that how they selected for a specific experiment. For example, in targeted drug delivery systems, nanoparticles act as good carriers. So according to nanoparticles characteristics and protein features in target cells, tissues and organisms nanoparticle-protein interactions could be predicted and then experimentally tested with respect to decrements in probability errors.
Keywords: Thermodynamic interaction, Corona, Free Gibbs energy, Protein fibrillation
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Motevalli, S.M., and Mirzajani, F., The Study of Nanoparticle Size and Coating Influence on the Protein Corona Thermodynamic, Int. J. Pure App. Biosci. 3(6): 42-58 (2015). doi: http://dx.doi.org/10.18782/2320-7051.1891
