International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2015, Volume : 3, Issue : 6
First page : (179) Last page : (182)
Article doi: http://dx.doi.org/10.18782/2320-7051.2172

Isolation, Identification and Screening of Cholesterol Degrading Probiotics

K. Vaishnavi, M. Krishma and P. Rajeswari*
The Standard Fireworks Rajaratnam College for Women, Sivakasi, Tamilnadu
*Corresponding Author E-mail: sholarrpl@gmail.com
Abstract
In this study, Cholesterol degrading Lactobacillus was isolated from butter milk sample.  The growth performance of Lactobacillus on MRS agar was identified by the colonies were formed due to the utilization of Lactose and by various biochemical tests. Lactobacilli are resistant to various antibiotics and bile salt. Growth performance of selected probiotic in various pH (such as 4, 7 & 9), Temperature (such as 40C, 370C, 500C), and various concentration of Bile salt (such as 0.1g, 0.2g, 0.3g,0.4g and 0.5g ) was detected. In vitro study of cholesterol degradation by probiotic was carried out and it was based on the growth performance and percentage of cholesterol removed from liquid cholesterol. The result of in vitro study reveals Lactobacillus showed the better degradation (21.5%) of cholesterol degraded in liquid cholesterol (at 600 µg/ml).

Key words: Cholesterol, Lactobacillus, Probiotics, Bile salt and MRS medium.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vaishnavi, K., Krishma, M. and Rajeswari, P., Isolation, Identification and Screening of Cholesterol Degrading Probiotics, Int. J. Pure App. Biosci. 3(6): 179-182 (2015). doi: http://dx.doi.org/10.18782/2320-7051.2172