International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2015, Volume : 3, Issue : 5
First page : (50) Last page : (55)
Article doi: http://dx.doi.org/10.18782/2320-7051.2138
Effect of Growth Regulators on In vitro Cultures of Aconitum heterophyllum: An Endangered Medicinal Plant
Ritu Mahajan*, Nisha Kapoor and Iqbal Singh
School of Biotechnology, University of Jammu, Jammu
*Corresponding Author E-mail: ritufeb@gmail.com
Abstract
Aconitum heterophyllum Wall. an endangered medicinal plant contains alkaloids that have significant antipyretic and analgesic role. An efficient protocol was developed for its mass shoot multiplication which can meet the requirements of pharmaceutical industries. The most effective treatment for sterilization of explant was three step sterilization method where 70% ethanol for 30 sec + HgCl2 (0.1%) for 2 min + KMnO4 (1ppm) for 3 min was used, leading to 73.3% of uncontaminated explants. The MS medium supplemented with 1.0mg/l BAP, 0.5mg/l Kn and 1.0mg/l GA3 resulted in shoot growth of 46.50% with an average number of shoots 2.7± 0.48 and average shoot length 2.63± 0.35. Six different combinations were used for callus induction and high frequency of embryogenic callus was obtained in MS medium supplemented with 2.0mg/l2,4-D.
Key words: Aconitum heterophyllum, in vitro, growth regulators, callus, endangered
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Mahajan, R., Kapoor, N. and Singh, I., Effect of Growth Regulators on In vitro Cultures of Aconitum heterophyllum: An Endangered Medicinal Plant, Int. J. Pure App. Biosci. 3 (5): 50-55 (2015). http://dx.doi.org/10.18782/2320-7051.2138
