International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2015, Volume : 3, Issue : 5
First page : (159) Last page : (168)
Article doi: http://dx.doi.org/10.18782/2320-7051.2124

Parametric optimization of lactic acid production and its scale up using free and immobilized cells of Lactobacillus amylovorus NRRL B- 4542

Pyde Acharya Nagarjun*
Department of Microbiology, Osmania University, Hyderabad-500 007 (A.P) India
*Corresponding Author E-mail: anpyde@gmail.com
Abstract
In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum lactic acid production from Lactobacillus amylovorus NRRL B4542 under submerged fermentation conditions. Various parameters viz. agitation, pH of medium, inoculum size, temperature, buffering agents and different starchy sources were taken into consideration. The optimum conditions were agitation (200rpm), pH (8.5), temperature (40oC), incubation time (30oC) and inoculum (7%) for maximum lactic acid production. Also, scale up studies were conducted using corn starch from free and calcium alginate entrapped cells of L. amylovorous NRRL B- 4542 on 15 L fermenter scale using corn starch (60 g/l) as carbon source. Free cells of L. amylovorous NRRL B-4542 showed lactic acid production (55g/l) and immobilized cells produced lactic acid (60g/l) with the maximum yield 0.96g/g after 3 cycles thereafter showed declination in lactic acid yield.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Nagarjun, P.A., Parametric optimization of lactic acid production and its scale up using free and immobilized cells of Lactobacillus amylovorus NRRL B- 4542, Int. J. Pure App. Biosci. 3 (5): 159-168 (2015). http://dx.doi.org/10.18782/2320-7051.2124