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Five different cytokinins effect on shoot regeneration from leaf segments culture of Cucumis trigonus was
studied. Cytokinins 6-benzyladenine (BA), kinetin (kn), zeatin, dimethyl allyl amino purine (DAAP) and
thidiazuron (TDZ) were used for direct shoot induction. Of the various cytokinins tested highest
regenerative response was observed in leaf segments cultured in Murashige and Skoog’s (MS) medium
supplemented with 1.0 mg L-1 BA + 0.25 mg L-1 indole-3-acetic acid (IAA), where all the explants
responded producing an average of 14.29 shoot buds per explant over a period of 6 weeks culture.
Differentiation and elongation of shoot buds was achieved by sub-culturing them on MS augmented with
5.0 mg L-1glutamine. Regenerated shoots were readily rooted in full and half strength MS medium and
about 95% of the plantlets survived on transfer to outdoors.
Key words: axenic leaves, Cucumis trigonus, cytokinins, glutamine, indole-3-acetic acid.