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Sunflower (Helianthus annuus L , is the most important oilseed crop, where salinity is an increasing
problem. Sunflower is moderately sensitivity. The physiological results showed that the effect of sodium
chloride was significant and highly significant decreased gradually for all varieties and all treatments, in
seeds germination (reached to 0% approximately in all varieties from 200 mM), roots lengths with a
difference of 22 cm from the control to 250 mM in Teddy bear variety. Concerning the biochemical effect,
the proteomic analysis using 2D-gel electrophoresis was limited to just for Teddy bear variety where
observed the existence of spots in plant control not observed in salt treated sample with 250 mM and
appearance others in sample treated not observed in the control sample. The SDS-PAGE for four
untreated varieties (Sakha53, Giza 102, Abendsoone and Teddy bear) showed (76.92%) of polymorphism,
while for untreated and treated varieties with 100 mM and 250 mM recorded (65%). The cluster analysis
for untreated varieties included the Egyptian varieties Sakha 53 and Giza 102 with (80%) similarity and
50% between Abendsoone and Teddy bear varieties, for untreated with treated varieties recorded
(72.7%) between all controls and 250 mM, while noted (25%) between Abendsoone variety and 250 mM,
while The effect of salt were positive with appearance of two bands (in 250 mM before and after 18.5 kDa
for Abendsoone variety) not observed in control.
Key words: Sunflower, Salinity, sodium chloride, 2D gel electrophoresis, SDS-PAGE, varieties.