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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2013 , Volume 1, Issue 6
Page No. : 51-55
Article doi: : http://dx.doi.org/10.18782
An efficient protocol for in vitro aseptic shoot multiplication and plant regeneration of Rosmarinus officinalis- An Important Medicinal Plant Using axillary bud
Leelavathi.D1 , Yashoda2 and Narendra Kuppan3*
1Head of Department of Botany, MES Degree College of Science, Bangalore
2Global Scientific Research Foundation, Mathekere, Bangalore, INDIA
3DOS in Botany, University of Mysore, Mysore
*Corresponding Author E-mail: narendrakuppan@gmail.com
ABSTRACT
The prime objective of the present investigation was to build up a repeatable protocol for rapid clonal
multiplication using in vitro axillary bud of Rosmarinus officinalis. In vitro axillary buds were
cultured on MS basal medium supplemented with BAP (8.88 μΜ) + IAA (2.85 μΜ) to induce multiple
shoots. Further, these shoots were subcultured on the same medium to produce more number of
multiple shoots. Well developed multiple shoots developed roots, when inoculated on rooting medium
on MSBM fortified with BAP (8.88μΜ) + NAA (2.68μΜ) + IBA (4.92μΜ) after 28 days of culture
(Fig.6) and the axenic plants are subjected to hardening (Fig.7). The acclimatized plants were
transferred to soil with 75% survival frequency.
Keywords: Rosmarinus officinalis L, axillary bud Murashige and Skoog Basal Medium.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 1 (6): 51-55 (2013)
