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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 4
Page No. : 276-279
Article doi: : http://dx.doi.org/10.18782
In vitro propagation of Indigofera trita L. F-Highly reputed medicinal plants
Senthilkumar M.1*, VinothkumarD.2, Anupama M.3 and Anandakumar D.4
1Department of Botany, Government Arts College, Dharmapuri, Tamilnadu, India
2Department of Biotechnology, Adhiyamaan Engineering College, Hosur, Tamil Nadu, India
3Department of Botany, Government Arts College, Karur, Tamilnadu, India
4Department of Botany, Government Arts College, Dharmapuri, Tamilnadu, India
*Corresponding Author E-mail: senthil2323@gmail.com
ABSTRACT
Protocol for rapid in vitro propagation of Indigofera trita through nodal explants multiplication was
established. Murashige and Skoog (MS) medium with 2 mg/l N-benzyladenine (BA) was best suited
for nodal proliferation. Excision and culture of the nodal segments from the In vitro shoots on fresh
medium with same concentrations of BA facilitated development of more than 20 shoots / node.
Subsequent culturesenhanced the rate of shoot proliferation. Shoots developed were rooted best on
half strength MS with 2 mg/l IBA. Starting from a single node explants, 150 rooted shoots were
obtained within 125 days. Plantlets established in pots exhibited 75% survival. Micropropagated
plants established in garden were uniform and identical to the donor plant with respect to
morphological and cytological characteristics.
Key words: Indigoferatrita,In vitro, plantlet production, nodal explants.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (4): 276-279 (2015)
