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Two pure breeds of mulberry silkworm namely Pure Mysore and NB4D2, at the age of fifth instar first day
were treated with different doses of ethyl methanesulfonate (EMS) by oral injection. The treated larvae
along with their respective controls were allowed to continue development. The F1 progenies were raised
by selfing the moths emerged from the ethyl methanesulfonate treated and control sets separately. The
control as well as F1 progeny raised from the treated silkworm larvae were collected daily with a regular
interval of 24 h. for haemolymph collection. The collected haemolymph was subjected for glucose assay.
The experimental results clearly indicated the significant variation in blood glucose level between control
and experimental sets as well as among experimental sets.
Key words: Silkworm, Bombyx mori, ethyl methanesulfonate, mutation, inheritance, haemolymph glucose
level.